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ELC-03XS – Universal Amplifier

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Versatile All-in-One Amplifier

  • Get one amplifier for multiple uses
  • Recording, staining, electroporation – no need to change pipette or headstage
  • Only amplifier on the market with this multifunctional use
  • Record in the low pA range and have headroom for up to 1.2 µA
  • A simpler version of this amplifier is the ELC-01X
  • Similar amplifier without voltage clamp is the BA-03X
  • Combine with our miniature headstages to recording in freely moving animals
  • Check out our tutorial videos about the ELC-03XS on YouTube
  • Book a demo of the ELC-03XS
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Features

  • Recording with patch, sharp, metal or carbon fiber electrodes
  • In vivo recordings with miniature headstages
  • Extracellular recordings with high gain
  • Headstages with additional extracellular channels
  • Juxtasomal filling of dyes or DNA
  • Intracellular recording in CC (bridge) mode with patch or sharp electrodes
  • Intracellular recording in VC mode with patch electrodes
  • Single cell stimulation
  • Amperometry, voltammetry and iontophoresis
  • Improved series resistance compensation
  • Telegraph lines for filters and gains
  • Reliable recording from astrocytes
  • Compatible to all major data acquisition systems
  • Combined in vivo whole-cell patch-clamp recordings with extracellular multielectrode recordings with the NEURALYNX system

Ordering

Part no. Description
ELC-03XS Universal Amplifier
ELC-MOD Cell Model Circuit for ELC System (passive)
ELC-SEAL Surplus for Seal Resistance Test
ELC-PRS Remote Switch for Penetration Unit
ELC-EH-SET Electrode Holder Set for ELC Amplifiers
ELC-EH Standard Micro-/Patch Electrode Holder
ELC-HS ELC Headstage
ELC-DIFF Surplus for Differential Headstage for ELC Amplifiers
ELC-SWI Surplus for switchable Headstage, x1/x100
ELC-MINI Miniature headstage for ELC-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options.

Please contact us for further information and prices.

 

Technical Data

Headstage

Input voltage range: ±12 V
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Dovetail: Size: 70 mm x 17 mm x 3 mm
Electrode connector: BNC with driven shield
REF connector: SMB connector
Ground connector: 2.4 mm connector
Input resistance (CC): >1013 Ω (internally adjustable)
Current range: ±100 nA max. (100 MΩ feedback, x1 range)
±1.0 µA max. (10 MΩ feedback, x10 range)
±10 µA max. (1 MΩ feedback, x100 range)
±10 nA max. (1 GΩ feedback, x0.1 range)

Electrode parameter controls

OFFSET: range ±100 mV, ten-turn control
PIPETTE HOLD POTENTIAL (in VC): range ±100 mV, ten-turn control
CAPACITY COMPENSATION: range 0 – 30 pF, ten-turn control
BIAS: range ±100 pA, ten-turn control
BRIDGE BALANCE: 0-100 MΩ, adjustable with ten-turn control

Electrode resistance test

Sensitivity 1 mV / MΩ application of square current pulses ±1 nA
Display: 3 ½ digit, XXX MΩ, activated by key switch
(same as POTENTIAL display)

Bandwidth and speed response (CC mode, optimal capacity compensation)

Full power bandwidth (REL = 0 MΩ): >30 kHz
rise time (10% – 90%) <10 µs (REL = 100 MΩ)
<5 µs (REL = 10 MΩ)

Outputs

Output impedance: 50 Ω
Max. voltage: ±12 V
Current output: BNC connector, sensitivity 0.1…10 V/nA,
Current output sensitivity: Rotary switch, 0.1, 0.2, 0.5, 1, 2, 5, 10 V/nA
Current display: 3 ½ digits, XX.XX nA, resolution 10 pA
Current LP filter: 4-pole BESSEL filter (other options available)
attenuation: -24 dB/octave
corner frequencies (Hz): 20, 50, 100, 200, 300, 500, 700, 1k, 1,3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k
Potential output x1: BNC connector, sensitivity 1 V/V
Potential output: BNC connector, sensitivity 10…1k V/V
Potential output gain: Rotary switch, 10, 20, 50, 100, 200, 500, 1k
Potential output resolution in AC: 50 µV
Potential LP filter: 4-pole BESSEL filter (other options available)
attenuation: -24 dB/octave
corner frequencies (Hz): 20, 50, 100, 200, 300, 500, 700, 1k, 1,3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k
Potential HP filter: 1-pole filter, (other options available)
attenuation: -6 dB/octave
corner frequencies (Hz): DC, 0.1, 0.3, 0.5, 1, 3, 5, 10, 30, 50, 100, 300, 500, 800, 1k, 3k
Telegraph potential LP filter -8…+7 V, 1V/step
Telegraph potential HP filter -8…+7 V, 1V/step
Telegraph current filter -8…+7 V, 1V/step
Telegraph potential output sensitivity +1…+7 V, 1V/step
Telegraph current output sensitivity +1…+7 V, 1V/step

Digital displays:

Display mV/MΩ 3 ½ digits, XXXX mV or XXX MΩ
Display current 3 ½ digits, XX.XX nA

Inputs

Input impedance analog 100 kΩ
Input range ±12 V
Input impedance digital (TTL) 10 kΩ
Input range TTL 0-5 V
Current stimulus input CC via BNC connectors, sensitivity 1 nA / V
Current stimulus input CCx10 via BNC connectors, sensitivity 10 nA / V
Step gate input via BNC connector (TTL)
Gated stimulus CC with ten-turn control of holding current
resolution: 10 pA, range: ±10 nA
Gated stimulus CCx10 with ten-turn control of holding current
resolution: 100 pA, range: ±100 nA
Polarity selectable with toggle switch
Voltage command input VC via BNC connectors, sensitivity: ÷10 mV
Voltage command input VCx10 via BNC connectors, sensitivity: ÷1 mV
Step gate input via BNC connector (TTL)
Gated stimulus VC with ten-turn control of holding potential
resolution: 1 mV, range: ±1 V
Gated stimulus VCx10 with ten-turn control of holding potential
resolution: 10 mV, range: ±10 V
Polarity selectable with toggle switch

Physical specifications

Dimensions: 19” rackmount cabinet
19” (483 mm), 10” (250 mm), 3.5” (88 mm)
Power requirements: 115/230 V AC, 60/50 Hz, fuse 0.4/0.2 A, slow, 25 W
Weight: 5.0 kg

Downloads

Manuals ELC-03XS_manual
Brochure ELC-Series

 

ABOUT

ELC amplifiers are multi technique systems where researchers can combine traditional patch and microelectrode recording with electrical stimulation, dye application or single cell transfection. All ELC amplifiers enable an investigator to identify a single cell by its characteristic spike train and then electroporate or stimulate this cell in situ. Using the loosepatch clamp technique for recording and stimulation in slice or in in vivo preparations, allow investigations under more natural, i.e. non-invasive conditions. Experiments can be executed with minimal damage to individual cells including subcellular structures such as dendrites and axons. Since no gigaseal is required for these types of experiments, the same pipette can even be used for several cells. The ELC incorporates a unique headstage with a resistive-feedback circuit capable of functioning as follows:

  1. Patch Clamp headstage with pA sensitivity
  2. High-impedance electrometer with bridge balance capable of functioning as a conventional bridge amplifier
  3. Extracellular amplifier with highpass and lowpass filters and gain up to 1000

ELC amplifiers come in 2 versions:

  • ELC-01X and the ELC-01MX (modular version) include a simple voltage clamp function for approaching a cell and forming a seal.
  • ELC-03XS is a complete patch clamp amplifier for whole-cell and perforated patch with improved series resistance compensation based on abridge balance circuit.

Therefore, ELC-03XS amplifiers offer an unprecedented combination of capabilities with only one headstage compared to other commercial amplifiers with a more limited functional design. Researchers will find this amplifier an excellent and very cost effective solution for carrying out multiple recording techniques while using several stimulation protocols.

Please contact npi for any desired special design or customization. We can deliver the amplifier tailored to your needs.

The device can be adapted to directly replace the discontinued Dagan 3900A, the Dagan PC-ONE and especially the Dagan BVC-700A.

Features

Features

  • Recording with patch, sharp, metal or carbon fiber electrodes
  • In vivo recordings with miniature headstages
  • Extracellular recordings with high gain
  • Headstages with additional extracellular channels
  • Juxtasomal filling of dyes or DNA
  • Intracellular recording in CC (bridge) mode with patch or sharp electrodes
  • Intracellular recording in VC mode with patch electrodes
  • Single cell stimulation
  • Amperometry, voltammetry and iontophoresis
  • Improved series resistance compensation
  • Telegraph lines for filters and gains
  • Reliable recording from astrocytes
  • Compatible to all major data acquisition systems
  • Combined in vivo whole-cell patch-clamp recordings with extracellular multielectrode recordings with the NEURALYNX system

Ordering

Ordering

Part no. Description
ELC-03XS Universal Amplifier
ELC-MOD Cell Model Circuit for ELC System (passive)
ELC-SEAL Surplus for Seal Resistance Test
ELC-PRS Remote Switch for Penetration Unit
ELC-EH-SET Electrode Holder Set for ELC Amplifiers
ELC-EH Standard Micro-/Patch Electrode Holder
ELC-HS ELC Headstage
ELC-DIFF Surplus for Differential Headstage for ELC Amplifiers
ELC-SWI Surplus for switchable Headstage, x1/x100
ELC-MINI Miniature headstage for ELC-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options.

Please contact us for further information and prices.

 

Technical Data

Technical Data

Headstage

Input voltage range: ±12 V
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Dovetail: Size: 70 mm x 17 mm x 3 mm
Electrode connector: BNC with driven shield
REF connector: SMB connector
Ground connector: 2.4 mm connector
Input resistance (CC): >1013 Ω (internally adjustable)
Current range: ±100 nA max. (100 MΩ feedback, x1 range)
±1.0 µA max. (10 MΩ feedback, x10 range)
±10 µA max. (1 MΩ feedback, x100 range)
±10 nA max. (1 GΩ feedback, x0.1 range)

Electrode parameter controls

OFFSET: range ±100 mV, ten-turn control
PIPETTE HOLD POTENTIAL (in VC): range ±100 mV, ten-turn control
CAPACITY COMPENSATION: range 0 – 30 pF, ten-turn control
BIAS: range ±100 pA, ten-turn control
BRIDGE BALANCE: 0-100 MΩ, adjustable with ten-turn control

Electrode resistance test

Sensitivity 1 mV / MΩ application of square current pulses ±1 nA
Display: 3 ½ digit, XXX MΩ, activated by key switch
(same as POTENTIAL display)

Bandwidth and speed response (CC mode, optimal capacity compensation)

Full power bandwidth (REL = 0 MΩ): >30 kHz
rise time (10% – 90%) <10 µs (REL = 100 MΩ)
<5 µs (REL = 10 MΩ)

Outputs

Output impedance: 50 Ω
Max. voltage: ±12 V
Current output: BNC connector, sensitivity 0.1…10 V/nA,
Current output sensitivity: Rotary switch, 0.1, 0.2, 0.5, 1, 2, 5, 10 V/nA
Current display: 3 ½ digits, XX.XX nA, resolution 10 pA
Current LP filter: 4-pole BESSEL filter (other options available)
attenuation: -24 dB/octave
corner frequencies (Hz): 20, 50, 100, 200, 300, 500, 700, 1k, 1,3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k
Potential output x1: BNC connector, sensitivity 1 V/V
Potential output: BNC connector, sensitivity 10…1k V/V
Potential output gain: Rotary switch, 10, 20, 50, 100, 200, 500, 1k
Potential output resolution in AC: 50 µV
Potential LP filter: 4-pole BESSEL filter (other options available)
attenuation: -24 dB/octave
corner frequencies (Hz): 20, 50, 100, 200, 300, 500, 700, 1k, 1,3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k
Potential HP filter: 1-pole filter, (other options available)
attenuation: -6 dB/octave
corner frequencies (Hz): DC, 0.1, 0.3, 0.5, 1, 3, 5, 10, 30, 50, 100, 300, 500, 800, 1k, 3k
Telegraph potential LP filter -8…+7 V, 1V/step
Telegraph potential HP filter -8…+7 V, 1V/step
Telegraph current filter -8…+7 V, 1V/step
Telegraph potential output sensitivity +1…+7 V, 1V/step
Telegraph current output sensitivity +1…+7 V, 1V/step

Digital displays:

Display mV/MΩ 3 ½ digits, XXXX mV or XXX MΩ
Display current 3 ½ digits, XX.XX nA

Inputs

Input impedance analog 100 kΩ
Input range ±12 V
Input impedance digital (TTL) 10 kΩ
Input range TTL 0-5 V
Current stimulus input CC via BNC connectors, sensitivity 1 nA / V
Current stimulus input CCx10 via BNC connectors, sensitivity 10 nA / V
Step gate input via BNC connector (TTL)
Gated stimulus CC with ten-turn control of holding current
resolution: 10 pA, range: ±10 nA
Gated stimulus CCx10 with ten-turn control of holding current
resolution: 100 pA, range: ±100 nA
Polarity selectable with toggle switch
Voltage command input VC via BNC connectors, sensitivity: ÷10 mV
Voltage command input VCx10 via BNC connectors, sensitivity: ÷1 mV
Step gate input via BNC connector (TTL)
Gated stimulus VC with ten-turn control of holding potential
resolution: 1 mV, range: ±1 V
Gated stimulus VCx10 with ten-turn control of holding potential
resolution: 10 mV, range: ±10 V
Polarity selectable with toggle switch

Physical specifications

Dimensions: 19” rackmount cabinet
19” (483 mm), 10” (250 mm), 3.5” (88 mm)
Power requirements: 115/230 V AC, 60/50 Hz, fuse 0.4/0.2 A, slow, 25 W
Weight: 5.0 kg

Downloads

Downloads

Manuals ELC-03XS_manual
Brochure ELC-Series

 

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