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OctaFlow II - Software Controlled Perfusion System

OctaFlow II – Software Controlled Perfusion System

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OctaFlow II – Multi-function Perfusion System

  • Rapid flush mode for repeatable results
  • Programmable solution delivery
  • Keyboard control for fast reaction
Category:

Details

Each OctaFlow II ™system includes the following:

  • Magnetic stand(s) with poles
  • Valve manifold(s) with islotation or pinch valves and 5ml pressurized syringe reservoirs
  • Flush valve(s)
  • Quartz MicroManifold® perfusion outlet manifold(s) in various configurations (see below)
  • 230µm ID and 600µm OD Teflon tubing (FEP-1) and compression fittings (CF-1) for 1/16″ ID tubing (other sizes available – see below)
  • USB cable and software

Each OctaFlow II ™ system features a choice of valves:

  • Pinch valves : The 3-way normally open and normally closed pinch valves require minimal maintenance in long-term perfusion experiments and have response times of 15-20 ms.
  • Isolation valves : The 2-way normally closed isolation valves are optimized for high-speed solution exchange. These valves are completely isolated when closed and have response times of 3 to 4 ms.

Clippard isolation valve

 

Pinch valves

 

Additional options include:

  • FEP-2: Clear Teflon tubing with 380µm ID and 760µm OD
  • QMM-8: Quartz MicroManifold® 8 to 1 channel perfusion outlet manifold with 100µm or 200µm ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-16: Quartz MicroManifold® 16 to 1 channel perfusion outlet manifold with 100µm or 200µm ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-24: Quartz MicroManifold® 24 to 1 channel perfusion outlet manifold with 100µm or 200µm ID polyimide-coated quartz tubing and tip (other sizes available)

All of the Quartz MicroManifold® perfusion outlet manifolds are also available with an additional channel with 200µm ID tube for flushing dead space and/or with Teflon tubing installed.

Ordering

Part No.

Description
ALA OctaFlow08P/CL 8-channel system: magnetic stand with 1 pole, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, 8/1 channel perfusion outlet manifold with tubing, 3- way isolation flush valve, cables and software
ALA OctaFlow16P/CL/1 16-channel system: 2 magnetic stands with 1 pole each, valves and valve manifolds, 5ml pressurized reservoirs, compression fittings, 16/1 channel perfusion outlet manifold with tubing, 3- way isolation flush valve, cables and software
ALA OctaFlow16P/CL/2 16-channel system: 2 magnetic stands with 1 pole each, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, two 8/1-channel perfusion outlet manifolds with tubing, two 3-way isolation flush valves, cables and software
ALA OctaFlow24P/CL/1 24-channel system: 3 magnetic stands with 1 pole each, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, 24/1 channel perfusion outlet manifold with tubing, 3-way isolation flush valve, cables and software
ALA OctaFlow24P/CL/2 24-channel system: 3 magnetic stands with 1 pole each, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, one 16/1- and one 8/1- channel perfusion outlet manifolds with tubing, two 3-way isolation flush valves, cables and software
ALA OctaFlow24P/CL/3 24-channel system: 3 magnetic stands with 1 pole each, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, three 8/1-channel perfusion outlet manifolds with tubing, three 3-way isolation flush valves, cables and software
ALA 10mlRES 10ml pressurized reservoir assembly with 3-way luer valve
ALA 5mlRES 5ml pressurized reservoir assembly with 3-way luer valve
ALA PV Replacement pinch valve
ALA CL Replacement Clippard Isolation valve
ALA L3V Replacement 3-way isolation flush valve
ALA L3VB Replacement 3-way isolation flush valve with LED
ALA QMM-8/16/24/WT Quartz MicroManifold® perfusion outlet manifold with 100µm or 200µm ID (please specify) tubing and tip (other sizes available – please call or email to inquire)
ALA QMM-8/16/24/1WT Quartz MicroManifold® perfusion outlet manifold with 100µm or 200µm ID (please specify) tubing and tip (other sizes available – please call or email to inquire) and additional channel with 200µm ID tube for flushing dead space
ALA FEP-1 4m clear Teflon tubing with 230µm ID and 600µm OD (for 100µm ID QMM’s)
ALA FEP-2 4m clear Teflon tubing with 380µm ID and 760µm OD (for 200µm ID QMM’s)
ALA CF-1 Compression fitting for PE-10 & PE-20 and FEP-1 and FEP-2 tubing to 1/16″ ID tubing

 

Choose:
P – pinch valves
CL – isolation valves
8 – 8 to 1 channel perfusion outlet
16 – 16 to 1 channel perfusion outlet
24 – 24 to 1 channel perfusion outlet

Optional:
WT – manifold with Teflon tubing installed

Technical Data

Maximum number of perfusion valves 24
Suction developed via internal Venturi pump 199 mmHg nominal
Maximum number of valves per bank 8 valves/bank, up to 3 banks
Typical speed of pressure rise 520mmHg/sec
Maximum current output per valve 100mA/ 350mA isolation/pinch valve
Pressures selectable in mm/Hg in increments of 1 or other units selectable
Maximum voltage/valve 18V
Sample flow rate 1ml in 9 min. @ 520 mmHg with standard QMM
Maximum on time per valve no limit
Minimum on time per valve 7ms @ 12V & 1ms @ 18V
Standard QMM 8 tubes with 100μm ID, 1 tube with 200μm ID, 100μm ID tip
Maximum input pressure 3970mmHg – other units selectable
Tip sizes available 100, 150, 200 μm ID
Maximum pressure applied ~800mmHg
Maximum number of sequence steps 300
Power requirements 120/220V
Maximum number of sequence events with looping 30020
OctaFlow interface 19″x3.5″x10″, 10 lbs
48.26 cm × 8.89 cm × 25.4 cm, 4.5kg
Infinite Step Function in Repeat Mode
Programmable Voltage Range 1-22Volts/valve
USB 2.0 Support software is compatible with Windows 7 (64 bit) and Windows 10

 

ABOUT

Overview

The OctaFlow II ™ from ALA is our most advanced drug application device for electrophysiology and imaging research. This fast perfusion system is designed to meet the needs of basic science and drug discovery researchers. Software control of all perfusion parameters makes the OctaFlow II ™ superior to all other perfusion systems.

Key features of the OctaFlow II ™ include:

  • Choice of low-maintenance pinch valves or fast-response isolation valves
  • Valve-control of solution flow instead of motorized manipulation of barreled pipettes, minimizing sample losses following solution exchange
  • Keyboard solution selection, for exchange “on the fly”
  • Trigger in /out for synchronization with data acquisition systems
  • Preprogrammed solution delivery through software
  • Rapid flush mode for easy cleaning
  • Programmable pressurization of fluids for optimal solution flow
  • Programmable analog outputs for valve identification, step number, or pressure information to data acquisition

Each OctaFlow II ™ system allows for a choice of 8, 16 or 24 reservoirs, which can be configured in different modes, depending on the experiment(s). Constant perfusion can be achieved through the combination of compound application and wash solution for minimization of solution convergence in manifold. Periodic application of compounds can be achieved through specific focal application. U-tube and J-tube configurations are also possible with the Octaflow II™.

The OctaFlow II ™ software runs on Windows 7 (64-bit) or Windows 10 and communicates with the OctaFlow II ™ controller via USB 2.0. Sequence steps are loaded at USB 2.0 speeds for fast editing and executing of sequence files. The graphical interface is designed to control all perfusion parameters from a single window. It also allows you to create configuration files and sequence files and to save them for future use. The Cleaning Manager function in the software allows for fast cleaning of reservoirs, valves and tubing.

Details

Details

Each OctaFlow II ™system includes the following:

  • Magnetic stand(s) with poles
  • Valve manifold(s) with islotation or pinch valves and 5ml pressurized syringe reservoirs
  • Flush valve(s)
  • Quartz MicroManifold® perfusion outlet manifold(s) in various configurations (see below)
  • 230µm ID and 600µm OD Teflon tubing (FEP-1) and compression fittings (CF-1) for 1/16″ ID tubing (other sizes available – see below)
  • USB cable and software

Each OctaFlow II ™ system features a choice of valves:

  • Pinch valves : The 3-way normally open and normally closed pinch valves require minimal maintenance in long-term perfusion experiments and have response times of 15-20 ms.
  • Isolation valves : The 2-way normally closed isolation valves are optimized for high-speed solution exchange. These valves are completely isolated when closed and have response times of 3 to 4 ms.

Clippard isolation valve

 

Pinch valves

 

Additional options include:

  • FEP-2: Clear Teflon tubing with 380µm ID and 760µm OD
  • QMM-8: Quartz MicroManifold® 8 to 1 channel perfusion outlet manifold with 100µm or 200µm ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-16: Quartz MicroManifold® 16 to 1 channel perfusion outlet manifold with 100µm or 200µm ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-24: Quartz MicroManifold® 24 to 1 channel perfusion outlet manifold with 100µm or 200µm ID polyimide-coated quartz tubing and tip (other sizes available)

All of the Quartz MicroManifold® perfusion outlet manifolds are also available with an additional channel with 200µm ID tube for flushing dead space and/or with Teflon tubing installed.

Ordering

Ordering

Part No.

Description
ALA OctaFlow08P/CL 8-channel system: magnetic stand with 1 pole, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, 8/1 channel perfusion outlet manifold with tubing, 3- way isolation flush valve, cables and software
ALA OctaFlow16P/CL/1 16-channel system: 2 magnetic stands with 1 pole each, valves and valve manifolds, 5ml pressurized reservoirs, compression fittings, 16/1 channel perfusion outlet manifold with tubing, 3- way isolation flush valve, cables and software
ALA OctaFlow16P/CL/2 16-channel system: 2 magnetic stands with 1 pole each, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, two 8/1-channel perfusion outlet manifolds with tubing, two 3-way isolation flush valves, cables and software
ALA OctaFlow24P/CL/1 24-channel system: 3 magnetic stands with 1 pole each, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, 24/1 channel perfusion outlet manifold with tubing, 3-way isolation flush valve, cables and software
ALA OctaFlow24P/CL/2 24-channel system: 3 magnetic stands with 1 pole each, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, one 16/1- and one 8/1- channel perfusion outlet manifolds with tubing, two 3-way isolation flush valves, cables and software
ALA OctaFlow24P/CL/3 24-channel system: 3 magnetic stands with 1 pole each, valves and valve manifold, 5ml pressurized reservoirs, compression fittings, three 8/1-channel perfusion outlet manifolds with tubing, three 3-way isolation flush valves, cables and software
ALA 10mlRES 10ml pressurized reservoir assembly with 3-way luer valve
ALA 5mlRES 5ml pressurized reservoir assembly with 3-way luer valve
ALA PV Replacement pinch valve
ALA CL Replacement Clippard Isolation valve
ALA L3V Replacement 3-way isolation flush valve
ALA L3VB Replacement 3-way isolation flush valve with LED
ALA QMM-8/16/24/WT Quartz MicroManifold® perfusion outlet manifold with 100µm or 200µm ID (please specify) tubing and tip (other sizes available – please call or email to inquire)
ALA QMM-8/16/24/1WT Quartz MicroManifold® perfusion outlet manifold with 100µm or 200µm ID (please specify) tubing and tip (other sizes available – please call or email to inquire) and additional channel with 200µm ID tube for flushing dead space
ALA FEP-1 4m clear Teflon tubing with 230µm ID and 600µm OD (for 100µm ID QMM’s)
ALA FEP-2 4m clear Teflon tubing with 380µm ID and 760µm OD (for 200µm ID QMM’s)
ALA CF-1 Compression fitting for PE-10 & PE-20 and FEP-1 and FEP-2 tubing to 1/16″ ID tubing

 

Choose:
P – pinch valves
CL – isolation valves
8 – 8 to 1 channel perfusion outlet
16 – 16 to 1 channel perfusion outlet
24 – 24 to 1 channel perfusion outlet

Optional:
WT – manifold with Teflon tubing installed

Technical Data

Technical Data

Maximum number of perfusion valves 24
Suction developed via internal Venturi pump 199 mmHg nominal
Maximum number of valves per bank 8 valves/bank, up to 3 banks
Typical speed of pressure rise 520mmHg/sec
Maximum current output per valve 100mA/ 350mA isolation/pinch valve
Pressures selectable in mm/Hg in increments of 1 or other units selectable
Maximum voltage/valve 18V
Sample flow rate 1ml in 9 min. @ 520 mmHg with standard QMM
Maximum on time per valve no limit
Minimum on time per valve 7ms @ 12V & 1ms @ 18V
Standard QMM 8 tubes with 100μm ID, 1 tube with 200μm ID, 100μm ID tip
Maximum input pressure 3970mmHg – other units selectable
Tip sizes available 100, 150, 200 μm ID
Maximum pressure applied ~800mmHg
Maximum number of sequence steps 300
Power requirements 120/220V
Maximum number of sequence events with looping 30020
OctaFlow interface 19″x3.5″x10″, 10 lbs
48.26 cm × 8.89 cm × 25.4 cm, 4.5kg
Infinite Step Function in Repeat Mode
Programmable Voltage Range 1-22Volts/valve
USB 2.0 Support software is compatible with Windows 7 (64 bit) and Windows 10

 

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